| Объем | 100 мкл |
| Синонимы | CHEK1, Chk1, kinase Chk1, EC 2.7.11.1, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O14757 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine 296(I-F-S(p)-N-L) derived from Human Chk1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.<br/><br/>Sanchez Y., Science 277:1497-1501(1997).<br />Flaggs G., Curr. Biol. 7:977-986(1997).<br />Semba S., Int. J. Oncol. 16:731-737(2000).<br /><br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser296 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HUVEC cells treated with UV using Chk1 (Phospho-Ser296) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Наименование: Phospho-CHEK1 (Ser296) Antibody.