| Объем | 50 мкл |
| Синонимы | Interferon regulatory factor 3, IRF-3, IRF3 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | Q14653 |
| Иммуноген | A synthesized peptide derived from human Phospho-IRF3 (S386) |
| Видовая специфичность | Human |
| Применение | ELISA, WB, Recommended dilution: WB:1:500-1:5000 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Immunology |
| Аббревиатура | Interferon regulatory factor 3 |
| Примечание | Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages. |
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Western Blot
Positive WB detected in:Hela whole cell lysate(treated with Calyculin A or not)
All lanes:Phospho-IRF3 antibody at 1.03µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 KDa
Observed band size: 47 KDa
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Наименование: Phospho-IRF3 (S386) Antibody.