| Объем | 50 мкл |
| Синонимы | Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | P15056 |
| Иммуноген | A synthesized peptide derived from human Phospho-BRAF (T401) |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Cell Biology |
| Аббревиатура | Serine/threonine-protein kinase B-raf |
| Примечание | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in:A549 whole cell lysate(treated with EGF or not)
All lanes:Phospho-BRAF antibody at 1.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 KDa
Observed band size: 85 KDa
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Immunofluorescence staining of HepG2 cells with CSB-RA002791A401phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Наименование: Phospho-BRAF (T401) Antibody.