| Объем | 100 мкг |
| Синонимы | Platelet endothelial aggregation receptor 1 (hPEAR1) (Multiple epidermal growth factor-like domains protein 12) (Multiple EGF-like domains protein 12), PEAR1, MEGF12 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q5VY43 |
| Иммуноген | Recombinant Human Platelet endothelial aggregation receptor 1 protein (932-1037AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Примечание | When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Platelet endothelial aggregation receptor 1 |
| Области исследований | Cardiovascular |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Rat liver tissue, Mouse kidney tissue
All lanes: PEAR1 antibody at 5.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 111 kDa
Observed band size: 111 kDa
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IHC image of CSB-PA017765LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of U251 cells with CSB-PA017765LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: PEAR1 Antibody.