| Объем | 100 мкл |
| Синонимы | AAPK1, AMPK alpha-1 chain, AMPK-alpha1, HMG-CoA redustase kinase, PRKAA1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13131/P54646 |
| Иммуноген | Peptide sequence around aa.485~489 (S-G-S-V-S) derived from Human AMPKa1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 |
| Примечание | Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. <br/><br/>Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.<br/>Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Области исследований | Neuroscience,Cancer,Cardiovascular,Metabolism,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells using AMPKa1(Ab-487)Antibody.
 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKa1(Ab-487)Antibody(left) or the same antibody preincubated with blocking peptide(right).
 |
| | |
Наименование: PRKAA1/PRKAA2 (Ab-487) Antibody.