PDPK1 (Ab-241) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | PDPK1, PkB kinase, Protein kinase B kinase, hPDK1, kinase PDK1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | O15530 | | Иммуноген | Peptide sequence around aa.239~243 (A-N-S-F-V) derived from Human PDK1. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development <br/><br/>Scheid MP,et al. (2005)Mol Cell Biol, 25(6): 2347-63<br/>Chen H, et al. (2001) Biochemistry, 40(39): 11851-9<br/>Sato S,et al. (2002) J Biol Chem, 277(42): 39360-7<br/>Lim MA, et al.(2003)Proc Natl Acad Sci U S A, 100(24): 14006-11<br/>Beausoleil SA, et al. (2004)Proc Natl Acad Sci U S A, 101(33): 12130-5<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from MCF, 293 and PC12 cells using PDK1(Ab-241) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1(Ab-241) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed MCF7 cells using PDK1(Ab-241) Antibody.
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Наименование: PDPK1 (Ab-241) Antibody.