| Объем | 50 мкг |
| Синонимы | Proliferating cell nuclear antigen (PCNA) (Cyclin), PCNA |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P12004 |
| Иммуноген | Recombinant Human Proliferating cell nuclear antigen protein (1-261AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Proliferating cell nuclear antigen |
| Области исследований | Isotype/Loading Controls, Epigenetics and Nuclear Signaling, Cancer, Cell biology, Tags & Cell Markers |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA01567A0Rb at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA01567A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, Raji whole cell lysate
All lanes: PCNA antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29, 25 kDa
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Наименование: PCNA Antibody.