PCNA AntibodyСпецификация| Объем | 100 мкл | | Синонимы | cyclin, proliferating cell nuclear antigen, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P12004 | | Иммуноген | Synthesized peptide derived from human PCNA. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 | | Примечание | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<br/><br/>Anje Sporbert, Nucleic Acids Res., Jun 2005, 33: 3521 - 3528. <br/>Sean C. Gehen, Am J Physiol Lung Cell Mol Physiol, Mar 2007, 292: L716 - L724. <br/>Basavaraj Bagewadi, J. Virol., Nov 2004, 78: 11890 - 11903. <br/>T Iida, I Suetake, Genes Cells, Oct 2002, 7: 997 - | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Isotype/Loading Controls,Epigenetics and Nuclear Signaling,Cancer,Cell biology,Tags & Cell Markers | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PCNA antibody.
 | Western blot analysis of extracts from HepG2 cells, using PCNA antibody.
 | Western blot analysis of extracts from Hela cells (Lane 2), colo cells(Lane 3), HepG2 cells (Lane 4) and 293 cells (Lane 5) using PCNA antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: PCNA Antibody.