| Объем | 50 мкг |
| Синонимы | Rho guanine nucleotide exchange factor 18 (114 kDa Rho-specific guanine nucleotide exchange factor) (p114-Rho-GEF) (p114RhoGEF) (Septin-associated RhoGEF) (SA-RhoGEF), ARHGEF18, KIAA0521 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q6ZSZ5 |
| Иммуноген | Recombinant Human Rho guanine nucleotide exchange factor 18 protein (159-551AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, Recommended dilution: WB:1:1000-1:5000 |
| Примечание | Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RgoGEF cts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. It may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS), but does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Rho guanine nucleotide exchange factor 18 protein |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Rho guanine nucleotide exchange factor 18 antibody at 2µg/ml
Lane 1: EC109whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 131, 115 kDa
Observed band size: 30, 60 kDa
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Наименование: ARHGEF18 Antibody.