Объем | 100 мкл |
Синонимы | KS6B1, P70-S6K, RPS6KB1, S6K, |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P23443 |
Иммуноген | Peptide sequence around phosphorylation site of serine 411 (I-R-S(p)-P-R) derived from Human p70 S6 Kinase. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
Примечание | Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.<br/><br/>Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851<br/>Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.<br/>Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c<br/>Le, X.F, et al. (2003) Oncogene 22: 484 |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Ser411 |
Области исследований | Epigenetics and Nuclear Signaling,Cell biology,Metabolism,Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase(Phospho-Ser411) Antibody.
![](http://www.cusabio.cn/images/cb/CSB-PA177270-WB-1.jpg) |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Ser411) Antibody(left) or the same antibody preincubated with blocking peptide(right).
![](http://www.cusabio.cn/images/cb/CSB-PA177270-IHC-1.jpg) |
Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase(Phospho-Ser411) Antibody.
![](http://www.cusabio.cn/images/cb/CSB-PA177270-IF-1.jpg) |
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