| Объем | 100 мкл |
| Синонимы | polypeptide 5, KS6A5, MSPK1, RPS6KA5, RLPK |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O75582 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 212 (A-Y-S(p)-F-C) derived from Human MSK1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | <p>Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).<br/><br/>McCoy CE, et al. (2007) Biochem J 402, 491-501 <br />McCoy CE, et al. (2005)Biochem J 387, 507-17 </p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser212 |
| Области исследований | Epigenetics and Nuclear Signaling,Cell biology,Metabolism,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from NIH-3T3 cells treated with EGF using MSK1 (Phospho-Ser212) antibody.The lane on the right is treated with the antigen-specific peptide.
 |
| | |
Наименование: Phospho-RPS6KA5 (Ser212) Antibody.