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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

NOP53 Antibody

Спецификация

Объем100 мкг
СинонимыRibosome biogenesis protein NOP53 (Glioma tumor suppressor candidate region gene 2 protein) (Protein interacting with carboxyl terminus 1) (PICT-1) (p60), NOP53, GLT GLTSCR2 PICT1
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotQ9NZM5
ИммуногенRecombinant Human Ribosome biogenesis protein NOP53 protein (227-405AA)
ИсточникRabbit
Видовая специфичностьHuman, Rat
ПрименениеELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000
ПримечаниеNucleolar protein which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis (PubMed:24120868). In ribosome biogenesis, may also play a role in rRNA transcription (PubMed:27729611). Also functions as a nucleolar sensor that regulates the activation of p53/TP53 in response to ribosome biogenesis perturbation, DNA damage and other stress conditions (PubMed:21741933, PubMed:24120868, PubMed:27829214). DNA damage or perturbation of ribosome biogenesis disrupt the interaction between NOP53 and RPL11 allowing RPL11 transport to the nucleoplasm where it can inhibit MDM2 and allow p53/TP53 activation (PubMed:24120868, PubMed:27829214). It may also positively regulate the function of p53/TP53 in cell cycle arrest and apoptosis through direct interaction, preventing its MDM2-dependent ubiquitin-mediated proteasomal degradation (PubMed:22522597). Originally identified as a tumor suppressor, it may also play a role in cell proliferation and apoptosis by positively regulating the stability of PTEN, thereby antagonizing the PI3K-AKT/PKB signaling pathway (PubMed:15355975, PubMed:16971513, PubMed:27729611). May also inhibit cell proliferation and increase apoptosis through its interaction with NF2 (PubMed:21167305). May negatively regulate NPM1 by regulating its nucleoplasmic localization, oligomerization and ubiquitin-mediated proteasomal degradation (PubMed:25818168). Thereby, may prevent NPM1 interaction with MYC and negatively regulate transcription mediated by the MYC-NPM1 complex (PubMed:25956029). May also regulate cellular aerobic respiration (PubMed:24556985). In the cellular response to viral infection, may play a role in the attenuation of interferon-beta through the inhibition of DDX58/RIG-1 (PubMed:27824081).
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки>95%, Protein G purified
АбревеатураRibosome biogenesis protein NOP53
Области исследованийEpigenetics and Nuclear Signaling, Cancer
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Rat heart tissue
All lanes: NOP53 antibody at 4.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa

IHC image of CSB-PA885778LA01HU diluted at 1:600 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:100 мкг 
Кат. номер:CSB-PA885778LA01HU
Цена (с НДС 20%):по запросуВ корзину
NOP53 AntibodyНаименование: NOP53 Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43