| Объем | 100 мкл |
| Синонимы | Factor A, GSK-3 alpha, kinase GSK3-alpha |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P49840 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3a. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. <br /><br />Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178. <br />Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760. <br />Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279. <br />Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser21 |
| Области исследований | Cardiovascular,Cell biology,Signal transduction,Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3a(Phospho-Ser21) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3a(Phospho-Ser21) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3a(Phospho-Ser21) Antibody.
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Western blot analysis of extracts from 293 cells, treated with insulin or calf intestinal phosphatase (CIP), using GSK3? (Phospho-Ser21) Antibody.
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Наименование: Phospho-GSK3A (Ser21) Antibody.