MSH2 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | COCA1, DNA mismatch repair protein Msh2, FCC1, HNPCC, HNPCC1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P43246 | | Иммуноген | Synthesized peptide derived from internal of human MSH2. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.<br/><br/>Fishel R., Cell 75:1027-1038(1993).<br/>Wang Y., Genes Dev. 14:927-939(2000).<br/>Wang Y., Proc. Natl. Acad. Sci. U.S.A. 100:15387-15392(2003).<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemistry analysis of paraffin-embedded human brain tissue using MSH2 antibody.
 | Immunofluorescence analysis of HUVEC cells, using MSH2 antibody.
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Наименование: MSH2 Antibody.