| Объем | 100 мкг |
| Синонимы | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (Complex I-B17) (CI-B17) (NADH-ubiquinone oxidoreductase B17 subunit), NDUFB6 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O95139 |
| Иммуноген | Recombinant Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 protein (2-67AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Cell biology, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HEK293 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse spleen tissue, Mouse lung tissue, Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue, Mouse thymus tissue
All lanes: NDUFB6 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16, 14 kDa
Observed band size: 16, 14 kDa
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA015653LA01HU at dilution of 1:100
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Immunoprecipitating NDUFB6 in HEK293 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA015653LA01HU in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA015653LA01HU (4µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (20µg)
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Наименование: NDUFB6 Antibody.