| Объем | 100 мкг |
| Синонимы | MOB kinase activator 1A (Mob1 alpha) (Mob1A) (Mob1 homolog 1B) (Mps one binder kinase activator-like 1B), MOB1A, C2orf6 MOB4B MOBK1B MOBKL1B |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9H8S9 |
| Иммуноген | Recombinant Human MOB kinase activator 1A protein (2-216AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:1000, IF:1:200-1:500, IP1:200-1:2000 |
| Примечание | Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | MOB kinase activator 1A |
| Области исследований | Cancer, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: MOB kinase activator 1A antibody at 2µg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: Mouse small intestine tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 26, 17 kDa
Observed band size: 26 kDa
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Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA872519HA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: MOB1A antibody at 3.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
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IHC image of CSB-PA872519HA01HU diluted at 1:1000 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of HepG2 cells with CSB-PA872519HA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating MOB1A in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA872519HA01HU in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2 :CSB-PA872519HA01HU (5µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (20µg)
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Наименование: MOB1A Antibody.