| Объем | 50 мкл |
| Синонимы | ADP-ribosylation factor GTPase-activating protein 3 (ARF GAP 3), ARFGAP3, ARFGAP1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9NP61 |
| Иммуноген | Recombinant Human ADP-ribosylation factor GTPase-activating protein 3 protein (217-516AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ARFGAP3 antibody at 4µg/ml
Lane 1: HepG2 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: A549 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 57, 52 kDa
Observed band size: 57 kDa
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA885676ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA885676ESR1HU at dilution of 1:100
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Immunoprecipitating ARFGAP3 in HepG2 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA885676ESR1HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA885676ESR1HU (4µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (20µg)
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Наименование: ARFGAP3 Antibody.