| Объем | 100 мкл |
| Синонимы | MITF M1, Microphthalmia-associated transcription factor, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O75030 |
| Иммуноген | Synthesized non-phosphopeptide derived from human MITF around the phosphorylation site of serine 180/73 (P-N-S(p)-P-M). |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
| Примечание | Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.<br/><br/>J篓鐣 |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Области исследований | Epigenetics and Nuclear Signaling,Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry analysis of paraffin-embedded human skin tissue using MITF (Ab-180/73) antibody.
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Immunofluorescence analysis of HeLa cells, using MITF (Ab-180/73) antibody.
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Western blot analysis of extracts from HepG2 cells (lane 1) and COLO205 cells (lane 2), using MITF (Ab-180/73) antibody.
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Western blot analysis of extracts from HepG2 cells (Lane 2) and 3T3 cells (Lane 3), using MITF (Ab-180/73) antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: MITF (Ab-180/73) Antibody.