ООО «Лабораторная Диагностика»
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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

MOB1A Antibody

Спецификация

Объем50 мкг
СинонимыMOB kinase activator 1A (Mob1 alpha) (Mob1A) (Mob1 homolog 1B) (Mps one binder kinase activator-like 1B), MOB1A, C2orf6 MOB4B MOBK1B MOBKL1B
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotQ9H8S9
ИммуногенRecombinant Human MOB kinase activator 1A protein (2-216AA)
ИсточникRabbit
Видовая специфичностьHuman, Mouse
ПрименениеELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:1000, IF:1:200-1:500, IP1:200-1:2000
ПримечаниеActivator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки>95%, Protein G purified
АбревеатураMOB kinase activator 1A
Области исследованийCancer, Signal transduction
Ссылка на страницу на сайте производителяссылка
Western blot
All lanes: MOB kinase activator 1A antibody at 2µg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: Mouse small intestine tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 26, 17 kDa
Observed band size: 26 kDa

Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA872519HA01HU at dilution of 1:100
Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: MOB1A antibody at 3.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa

IHC image of CSB-PA872519HA01HU diluted at 1:1000 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-PA872519HA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating MOB1A in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA872519HA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2 :CSB-PA872519HA01HU (5µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (20µg)

  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:50 мкг 
Кат. номер:CSB-PA872519HA01HU
Цена (с НДС 20%):по запросуВ корзину
MOB1A AntibodyНаименование: MOB1A Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43