MDM2 (Ab-166) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | HDMX, hdm2 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q00987 | | Иммуноген | Peptide sequence around aa.164~168 (A-I-S-E-T) derived from Human MDM2. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | <p>This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues <br /><br />Haupt, Y. et al. (1997) Nature 387, 296-299. <br />Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. <br />Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415. <br />Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HT29, Hela and 293 cells using MDM2(Ab-166) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2(Ab-166) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using MDM2(Ab-166) Antibody.
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Наименование: MDM2 (Ab-166) Antibody.