ООО «Лабораторная Диагностика»
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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

LATS1 Antibody

Спецификация

Объем100 мкг
СинонимыSerine/threonine-protein kinase LATS1 (EC 2.7.11.1) (Large tumor suppressor homolog 1) (WARTS protein kinase) (h-warts), LATS1, WARTS
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotO95835
ИммуногенRecombinant Human Serine/threonine-protein kinase LATS1 protein (1-280AA)
ИсточникRabbit
Видовая специфичностьHuman
ПрименениеELISA, IHC, IF, IP, Recommended dilution: IHC:1:20-1:200, IF:1:50-1:500, IP:1:200-1:2000
ПримечаниеNegative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки>95%, Protein G purified
АбревеатураSerine/threonine-protein kinase LATS1
Области исследованийEpigenetics and Nuclear Signaling, Cancer, Cell biology, Signal transduction
Ссылка на страницу на сайте производителяссылка
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA012769LA01HU at dilution of 1:100
Immunofluorescent analysis of Hela cells using CSB-PA012769LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating LATS1 in K562 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA012769LA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA012769LA01HU (6µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)

IHC image of CSB-PA012769LA01HU diluted at 1:1200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA012769LA01HU diluted at 1:1200 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:100 мкг 
Кат. номер:CSB-PA012769LA01HU
Цена (с НДС 20%):по запросуВ корзину
LATS1 AntibodyНаименование: LATS1 Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43