| Объем | 100 мкг |
| Синонимы | NmrA-like family domain-containing protein 1, NMRAL1, HSCARG |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9HBL8 |
| Иммуноген | Recombinant Human NmrA-like family domain-containing protein 1 protein (1-299AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IF, Recommended dilution: IF:1:200-1:500 |
| Примечание | Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescence staining of PC-3 cells with CSB-PA867194LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: NMRAL1 Antibody.