NFKB2 (Ab-872) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10, Lyt10, NFKB2 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q00653 | | Иммуноген | Peptide sequence around aa.870~874 (S-Q-S-V-E) derived from Human NFkB-p100. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | <p>NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. <br /><br />Shin HM, et al. (2006) EMBO J, 25(1): 129-138. <br />Li Q, et al. (2005) Proc Natl Acad Sci USA, 102(35): 12425-12430. <br />Chen C, et al. (2000) Mol Cell Biol, 20(8): 2687-2695. <br /> ,</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HT29 cells using NFkB-p100(Ab-872) Antibody and the same antibody preincubated with blocking peptide.
 | Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NFkB-p100/p52(Ab-872) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p100(Ab-872) Antibody.
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Наименование: NFKB2 (Ab-872) Antibody.