| Объем | 100 мкл |
| Синонимы | O-6-methylguanine-DNA methyltransferase |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P16455 |
| Иммуноген | Fusion protein of human MGMT |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB,ELISA:1:1000-1:2000, WB:1:200-1:1000 |
| Примечание | MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen affinity purification |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Gel: 10%SDS-PAGE, Lysate: 40 ?g, Lane: Raji cells, Primary antibody: CSB-PA821117(MGMT Antibody) at dilution 1/150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds
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Наименование: MGMT Antibody.