MDH1 AntibodyСпецификация| Объем | 100 мкг | | Синонимы | Malate dehydrogenase, cytoplasmic (EC 1.1.1.37) (Cytosolic malate dehydrogenase) (Diiodophenylpyruvate reductase) (EC 1.1.1.96), MDH1, MDHA | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P40925 | | Иммуноген | Recombinant Human Malate dehydrogenase, cytoplasmic protein (1-334AA) | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 | | Примечание | cytosol, extracellular exosome, extracellular space, mitochondrion, L-malate dehydrogenase activity, malic enzyme activity, gluconeogenesis, malate metabolic process, NADH metabolic process, oxaloacetate metabolic process | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | Malate dehydrogenase, cytoplasmic | | Области исследований | Cancer, Metabolism, Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | IHC image of CSB-PA013621EA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Western Blot
Positive WB detected in: HL60 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, LO2 whole cell lysate, NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse liver tissue
All lanes: MDH1 antibody at 2.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 28, 39 kDa
Observed band size: 37 kDa
 | Immunofluorescence staining of U251 cells with CSB-PA013621EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Информация для заказа |
Наименование: MDH1 Antibody.