| Объем | 100 мкг |
| Синонимы | Mannan-binding lectin serine protease 2 (EC 3.4.21.104) (MBL-associated serine protease 2) (Mannose-binding protein-associated serine protease 2) (MASP-2) [Cleaved into: Mannan-binding lectin serine protease 2 A chain, Mannan-binding lectin serine protease 2 B chain], MASP2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O00187 |
| Иммуноген | Recombinant Human Mannan-binding lectin serine protease 2 protein (189-314AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
| Примечание | Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Mannan-binding lectin serine protease 2 |
| Области исследований | Cardiovascular, Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: A549 whole cell lysate, SH-SY5Y whole cell lysate
All lanes: MASP2 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76, 21 kDa
Observed band size: 76 kDa
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IHC image of CSB-PA013510LA01HU diluted at 1:100 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: MASP2 Antibody.