| Объем | 50 мкл |
| Синонимы | Histone H2B type 1-K, H2B K, HIRA-interacting protein 1, HIST1H2BK, H2BFT, HIRIP1 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | O60814 |
| Иммуноген | A synthesized peptide |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:5000-1:10000, IHC:1:50-1:500 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Epigenetics and Nuclear Signaling |
| Аббревиатура | Histone H2B type 1-K |
| Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in:Mouse brain tissue,Rat liver tissue,Rat lung tissue
All lanes:Histone H2B type 1-K antibody at 0.16µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 KDa
Observed band size: 14 KDa
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IHC image of CSB-RA010411A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: Histone H2B type 1-K Antibody.