| Объем | 100 мкг |
| Синонимы | Galectin-1 (Gal-1) (14 kDa laminin-binding protein) (HLBP14) (14 kDa lectin) (Beta-galactoside-binding lectin L-14-I) (Galaptin) (HBL) (HPL) (Lactose-binding lectin 1) (Lectin galactoside-binding soluble 1) (Putative MAPK-activating protein PM12) (S-Lac lectin 1), LGALS1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P09382 |
| Иммуноген | Recombinant Human Galectin-1 protein (2-135AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Galectin-1 |
| Области исследований | Cancer, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Galectin-1 antibody at 2µg/ml
Lane 1: HL60 whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
 |
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA012882HA01HU at dilution of 1:100
 |
Immunofluorescent analysis of A549 cells using CSB-PA012882HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
 |
IHC image of CSB-PA012882HA01HU diluted at 1:400 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 |
| | |
Наименование: LGALS1 Antibody.