GAPDH AntibodyСпецификация| Объем | 100 мкл | | Синонимы | G3PD, GAPD, MGC88685 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P04406 | | Иммуноген | Peptide sequence around aa.252~256(P-A-K-Y-D) derived from Human GAPDH. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB,WB:1:500-1:1000 | | Примечание | <p>Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. <br /><br />Ercolani L., Florence B., Denaro M., Alexander M. J. Biol. Chem. 263:15335-15341(1988)<br />Tisdale E.J.J. Biol. Chem. 277:3334-3341(2002)</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Isotype/Loading Controls,Neuroscience,Cancer,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extract from Hela, 293 and 3T3 cells using GAPDH Antibody
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Наименование: GAPDH Antibody.