GFI1B AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Gfi-1b | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q5VTD9 | | Иммуноген | Peptide sequence around aa.322~326(H-R-E-S-Q) derived from Human GFI1b. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB,WB:1:500-1:1000 | | Примечание | <p>Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context, represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1, GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription, as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation. <br /><br />Osawa M., Yamaguchi T., Nakamura Y. Iwama A.Blood 100:2769-2777(2002) <br />Huang D.-Y., Kuo Y.-Y., Chang Z.-F. Nucleic Acids Res. 33:5331-5342(2005)<br />Vassen L., Fiolka K., Moeroey T. EMBO J. 25:2409-2419(2006)</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cardiovascular | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from Raji cells using GFI1b Antibody.
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Наименование: GFI1B Antibody.