| Объем | 100 мкг |
| Синонимы | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-), GAPDH, GAPD |
| Клональность | Polyclonal Antibody |
| Организм | Cricetulus griseus |
| uniprot | P17244 |
| Иммуноген | Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase protein (2-333AA) |
| Источник | Rabbit |
| Видовая специфичность | Cricetulus griseus |
| Применение | ELISA, WB, Recommended dilution: WB:1:500-1:5000 |
| Примечание | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Glyceraldehyde-3-phosphate dehydrogenase |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in Recombinant protein
All lanes: GAPDH antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
predicted band size: 36 kDa
observed band size: 36 kDa
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Наименование: GAPDH Antibody.