| Объем | 50 мкг |
| Синонимы | Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (EC 1.1.1.41) (Isocitric dehydrogenase subunit alpha) (NAD(+)-specific ICDH subunit alpha), IDH3A |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P50213 |
| Иммуноген | Recombinant Human Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial protein (28-108AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | mitochondrial matrix, mitochondrion, nucleus, carbohydrate metabolic process, tricarboxylic acid cycle |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial |
| Области исследований | Cancer, Tags & Cell Markers, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 whole cell lysat, Jurkat whole cell lysate, Rat brain tissue, Mouse brain tissue, Mouse liver tissue
All lanes: IDH3A antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40, 32 kDa
Observed band size: 40 kDa
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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA010991LA01HU at dilution of 1:100
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Immunofluorescent analysis of A549 cells using CSB-PA010991LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating IDH3A in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010991LA01HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010991LA01HU (6µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (20µg)
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Наименование: IDH3A Antibody.