ООО «Лабораторная Диагностика»
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/ Каталог / Реагенты для научных исследований / Антитела / Рекомбинантные антитела Cusabio

FEN1 Antibody

Спецификация

Объем50 мкл
СинонимыFlap endonuclease 1UniRule annotation, DNase IV, Flap structure-specific endonuclease 1UniRule annotation, FEN1
Тип антителRecombinant Antibody
SpeciesHuman
UniProt IDP39748
ИммуногенA synthesized peptide derived from human FEN1
Видовая специфичностьHuman
ПрименениеELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
КлональностьMonoclonal
ИзотипRabbit IgG
КоньюгатNon-conjugated
БуфферRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очисткиAffinity-chromatography
Области исследованийEpigenetics and Nuclear Signaling
АббревиатураFlap endonuclease 1
ПримечаниеStructure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
All lanes: FEN1 antibody at 0.775µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 36 KDa
Observed band size: 43 KDa

IHC image of CSB-RA008585A0HU diluted at 1:77.5 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-RA008585A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:50 мкл 
Кат. номер:CSB-RA008585A0HU
Цена (с НДС 20%):по запросуВ корзину
FEN1 AntibodyНаименование: FEN1 Antibody.
Примечание: дополнительная информация (на английском языке).
   
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