Histone H2A type 1-B/E AntibodyСпецификация| Объем | 100 мкл |  | Синонимы | Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, HIST1H2AB, H2AFM, AND, HIST1H2AE, H2AFA |  | Тип антител | Recombinant Antibody |  | Species | Human |  | UniProt ID | P04908 |  | Иммуноген | A synthesized peptide |  | Видовая специфичность | Human |  | Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:30-1:200 |  | Клональность | Monoclonal |  | Изотип | Rabbit IgG |  | Коньюгат | Non-conjugated |  | Буффер | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |  | Форма | Liquid |  | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |  | Метод очистки | Affinity-chromatography |  | Области исследований | Epigenetics and Nuclear Signaling |  | Аббревиатура | Histone H2A type 1-B/E |  | Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |  | Ссылка на страницу на сайте производителя | ссылка |  Western Blot 
Positive WB detected in:HepG2 whole cell lysate 
All lanes:Histone H2A type 1-B/E antibody at 2.7µg/ml 
Secondary 
Goat polyclonal to rabbit IgG at 1/50000 dilution 
Predicted band size: 13 KDa 
Observed band size: 13 KDa
 
   |  Immunofluorescence staining of Hela cells with CSB-RA010385A0HU at 1:168,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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 Информация для заказа| Область использования: | Производство: | Cusabio |  | Метод: | Антитела  |  | Объем: | 100 мкл  |  | Кат. номер: | CSB-RA010385A0HU |  | Цена (с НДС 20%): | по запросу | В корзину   |   Наименование: Histone H2A type 1-B/E Antibody. Примечание: дополнительная информация (на английском языке). |  
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