HAMP AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Hepcidin, Liver-expressed antimicrobial peptide 1, LEAP-1, Putative liver tumor regressor, PLTR, Hepcidin-25, Hepc25, Hepcidin-20, Hepc20, HAMP, HEPC, LEAP1, UNQ487/PRO1003 | | Тип антител | Recombinant Antibody | | Species | Human | | UniProt ID | P81172 | | Иммуноген | A synthesized peptide derived from human HAMP | | Видовая специфичность | Human | | Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200 | | Клональность | Monoclonal | | Изотип | Rabbit IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Affinity-chromatography | | Области исследований | Cardiovascular | | Аббревиатура | Hepcidin | | Примечание | Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005). | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate
All lanes: HAMP antibody at 2.15µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 10 KDa
Observed band size: 25 KDa
 | IHC image of CSB-RA010124A0HU diluted at 1:215 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: HAMP Antibody.