ESR1 (Ab-537) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | ER, ESR, ESR1, ESTR, ESTRA | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P03372 | | Иммуноген | Synthesized non-phosphopeptide derived from human Estrogen Receptor-alpha around the phosphorylation site of tyrosine 537 (P-L-Y(p)-D-L). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 | | Примечание | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements, the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Isoform 3 can bind to ERE and inhibit isoform 1.<br/><br/>Sehime Temel, Endocrinology, Oct 2002, 143: 3974. <br/>Xin-Tian Zhang, AACR Meeting Abstracts, Apr 2005, 2005: 302. <br/>Suwit J. Somponpun, Am J Physiol Regulatory Integrative Comp Physiol, Sep 2004, 287: R661 - R669.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 293cells, using Estrogen Receptor-? (Ab-537) antibody.
 | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-? (Ab-537) antibody.
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Наименование: ESR1 (Ab-537) Antibody.