| Объем | 100 мкл |
| Синонимы | ATP-dependent DNA helicase DDX11 (EC 3.6.4.12) (CHL1-related protein 1) (hCHLR1) (DEAD/H-box protein 11) (Keratinocyte growth factor-regulated gene 2 protein) (KRG-2), DDX11, CHL1 CHLR1 KRG2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q96FC9 |
| Иммуноген | Recombinant Human ATP-dependent DNA helicase DDX11 protein (1-220AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Probable ATP-dependent DNA helicase DDX11 |
| Области исследований | Epigenetics and Nuclear Signaling, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: DDX11 antibody at 8.47µg/ml
Lane 1: Mouse gonadal tissue
Lane 2: K562 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 109, 102, 99, 97, 33 kDa
Observed band size: 109 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA853416ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA853416ESR2HU at dilution of 1:100
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Наименование: DDX11 Antibody.