CDK4 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | PSK-J3, CMM3, CMM3 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P11802 | | Иммуноген | Peptide sequence around aa.295~299(L-H-K-D-E)derived from Human CDK4. | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | | Примечание | <p>Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle . <br /><br />Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681. <br />Sherr, C.J. (1996) Science 274, 1672-1677. <br />Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925. <br />Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Isotype/Loading Controls,Epigenetics and Nuclear Signaling,Cancer,Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HUVEC cells using CDK4 Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue usingCDK4 Antibody.
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Наименование: CDK4 Antibody.