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/ Каталог / Реагенты для научных исследований / Антитела / Моноклональные антитела Cusabio

CD146 Monoclonal Antibody

Спецификация

Объем50 мкл
Code NO#4D10C7
СинонимыCell surface glycoprotein MUC18 (Cell surface glycoprotein P1H12) (Melanoma cell adhesion molecule) (Melanoma-associated antigen A32) (Melanoma-associated antigen MUC18) (S-endo 1 endothelial-associated antigen) (CD antigen CD146), MCAM, MUC18
Тип антителMonoclonal Antibody
Видовая специфичностьHuman
Uniport IDP43121
ИммуногенRecombinant Human Cell surface glycoprotein MUC18 protein (50-646AA)
ИсточникMouse
Видовая специфичность 1Human
ПрименениеELISA, WB, IF, FC, Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200
ПримечаниеPlays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
ИзотипIgG2a
Метод очистки>95%, Protein G purified
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Области исследованийImmunology
Станица на сайте производителяссылка
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: CD146 antibody at 1:1250
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 72, 58 kDa
Observed band size: 120 kDa

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate
All lanes: CD146 antibody at 1:500
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 72, 58 kDa
Observed band size: 120 kDa

Immunofluorescence staining of A375 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of Hela cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of MCF-7 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescence staining of THP-1 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay histogram showing MCF-7 cells stained with CSB-MA013563A0m (red line) at 1:400. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:50 мкл 
Кат. номер:CSB-MA013563A0m
Цена (с НДС 20%):по запросуВ корзину
CD146 Monoclonal AntibodyНаименование: CD146 Monoclonal Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43