| Объем | 100 мкл |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P14635 |
| Иммуноген | Peptide sequence around aa.145~149 (A-F-S-D-V) derived from Human Cyclin B1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
| Примечание | <p>The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. <br /><br />Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470. <br />Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685. <br />Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HepG2 and HT29 cells using Cyclin B1(Ab-147) Antibody.
 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1(Ab-147) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 |
Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1(Ab-147) Antibody.
 |
| | |
Наименование: CCNB1 (Ab-147) Antibody.