CHUK (Ab-23) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | O15111 | | Иммуноген | Peptide sequence around aa.21~25 (L-G-T-G-G) derived from Human IKK a. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | | Примечание | Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. <br/><br/>Yuan ZQ, et al.(2002)J Biol Chem, 277(33): 29973-82.<br/>Ozes ON, et al. (1999)Nature, 401(6748): 82-5. <br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Cancer,Cardiovascular,Cell biology,Immunology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from Hela, 293 and 3T3 cells using IKK a(Ab-23) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK a(Ab-23) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: CHUK (Ab-23) Antibody.