CAMK4 AntibodyСпецификация| Объем | 50 мкл | | Синонимы | Calcium/calmodulin-dependent protein kinase type IV (CaMK IV) (EC 2.7.11.17) (CaM kinase-GR), CAMK4, CAMK CAMK-GR CAMKIV | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q16566 | | Иммуноген | Recombinant Human Calcium/calmodulin-dependent protein kinase type IV protein (304-473AA) | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IP, Recommended dilution: WB:1:1000-1:5000, IP:1:200-1:2000 | | Примечание | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen Affinity Purified | | Области исследований | Epigenetics and Nuclear Signaling, Neuroscience, Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot
All lanes: CAMK4 antibody at 2µg/ml + 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
 | Immunoprecipitating CAMK4 in Jurkat whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA614976ESR2HU in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA614976ESR2HU (4µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (20µg)
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Наименование: CAMK4 Antibody.