| Объем | 100 мкл |
| Синонимы | Cell division control protein 45 homolog (PORC-PI-1), CDC45, CDC45L CDC45L2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O75419 |
| Иммуноген | Recombinant Human Cell division control protein 45 homolog protein (1-270AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000, IF:1:50-1:200 |
| Примечание | Required for initiation of chromosomal DNA replication. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Cell division control protein 45 homolog |
| Области исследований | Epigenetics and Nuclear Signaling, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CDC45 antibody at 0.3µg/ml
Lane 1: K562 whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 66, 61, 69 kDa
Observed band size: 66, 69 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA005020ESR2HU at dilution of 1:100
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Immunoprecipitating CDC45 in K562 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA005020ESR2HU in K562 whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA005020ESR2HU (4µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (20µg)
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Immunofluorescent analysis of Hela cells using CSB-PA005020ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: CDC45 Antibody.