| Объем | 50 мкг |
| Синонимы | T-cell surface glycoprotein CD3 zeta chain (T-cell receptor T3 zeta chain) (CD antigen CD247), CD247, CD3Z T3Z TCRZ |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P20963 |
| Иммуноген | Recombinant Human T-cell surface glycoprotein CD3 zeta chain protein (88-153AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | T-cell surface glycoprotein CD3 zeta chain |
| Области исследований | Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: CD247 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
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Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA10499A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA10499A0Rb at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA10499A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating CD247 in jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA10499A0Rb in jurkat whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA10499A0Rb (8µg) + jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)
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Наименование: CD247 Antibody.