| Объем | 50 мкг |
| Синонимы | Caspase-2 (CASP-2) (EC 3.4.22.55) (Neural precursor cell expressed developmentally down-regulated protein 2) (NEDD-2) (Protease ICH-1) [Cleaved into: Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12], CASP2, ICH1 NEDD2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P42575 |
| Иммуноген | Recombinant Human Caspase-2 protein (2-452AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:1000-1:2000, IF:1:200-1:500 |
| Примечание | nvolved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Caspase-2 |
| Области исследований | Cancer, Cell biology, Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
IHC image of CSB-PA004547LA01HU diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-PA004547LA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: CASP2 Antibody.