| Объем | 50 мкг |
| Синонимы | Zinc-alpha-2-glycoprotein (Zn-alpha-2-GP) (Zn-alpha-2-glycoprotein), AZGP1, ZAG ZNGP1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P25311 |
| Иммуноген | Recombinant Human Zinc-alpha-2-glycoprotein (61-210AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Immunology, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: AZGP1 antibody at 4µg/ml
Lane 1: PC-3 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: LO2 whole cell lysate
Lane 4: 293T whole cell lysate
Lane 5: Hela whole cell lysate
Lane 6: HepG2 whole cell lysate
Lane 7: K562 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
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Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA11039A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA11039A0Rb at dilution of 1:100
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IHC image of CSB-PA11039A0Rb diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating NEFL in Mouse brain tissue
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA11039A0Rb in Mouse brain tissue.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA11039A0Rb (8µg) + Mouse brain tissue (500µg)
Lane 3: Mouse brain tissue (10µg)
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Наименование: AZGP1 Antibody.