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/ Каталог / Реагенты для научных исследований / Антитела / Рекомбинантные антитела Cusabio

Acetyl-Histone H2B type 1-B(K20)Antibody

Спецификация

Объем50 мкл
СинонимыHistone H2B type 1-B, Histone H2B.1, Histone H2B.f, H2B/f, HIST1H2BB, H2BFF
Тип антителRecombinant Antibody
SpeciesHuman
UniProt IDP33778
ИммуногенA synthesized peptide
Видовая специфичностьHuman, Mouse
ПрименениеELISA, WB, ICC, IF, FC, Recommended dilution: WB:1:5000-1:10000, ICC:1:50-1:500, IF:1:30-1:200
КлональностьMonoclonal
ИзотипRabbit IgG
КоньюгатNon-conjugated
БуфферRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очисткиAffinity-chromatography
Области исследованийEpigenetics and Nuclear Signaling
АббревиатураHistone H2B type 1-B
ПримечаниеCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min
All lanes:Acetyl-Histone H2B type 1-B(K20)antibody at 0.135µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 KDa
Observed band size: 15 KDa

Immunocytochemistry analysis of CSB-RA010402A20acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010402A20acHU at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay histogram showing Hela cells stained with CSB-RA010402A20acHU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:50 мкл 
Кат. номер:CSB-RA010402A20acHU
Цена (с НДС 20%):по запросуВ корзину
Acetyl-Histone H2B type 1-B(K20)AntibodyНаименование: Acetyl-Histone H2B type 1-B(K20)Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43