ООО «Лабораторная Диагностика»
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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

ATAT1 Antibody

Спецификация

Объем100 мкг
СинонимыAlpha-tubulin N-acetyltransferase 1 (Alpha-TAT) (Alpha-TAT1) (TAT) (EC 2.3.1.108) (Acetyltransferase mec-17 homolog), ATAT1, C6orf134 MEC17
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotQ5SQI0
ИммуногенRecombinant Human Alpha-tubulin N-acetyltransferase 1 protein (194-238AA)
ИсточникRabbit
Видовая специфичностьHuman, Rat, Mouse
ПрименениеELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500
ПримечаниеSpecifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics, this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPreservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки>95%, Protein G purified
АбревеатураAlpha-tubulin N-acetyltransferase 1
Области исследованийCell biology
Ссылка на страницу на сайте производителяссылка
Western Blot
Positive WB detected in: Rat kidney tissue, Mouse brain tissue, Mouse liver tissue
All lanes: ATAT1 antibody at 6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 46, 45, 38, 37, 36, 34 kDa
Observed band size: 47 kDa

IHC image of CSB-PA725542LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA725542LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:100 мкг 
Кат. номер:CSB-PA725542LA01HU
Цена (с НДС 20%):по запросуВ корзину
ATAT1 AntibodyНаименование: ATAT1 Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43