| Объем | 50 мкг |
| Синонимы | Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35), Anxa1, Anx1 Lpc-1 Lpc1 |
| Клональность | Polyclonal Antibody |
| Организм | Mouse |
| uniprot | P10107 |
| Иммуноген | Recombinant Mouse Annexin A1 protein (2-346AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IP, Recommended dilution: WB:1:1000-1:5000, IP:1:200-1:2000 |
| Примечание | Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Anxa1 antibody at 2µg/ml
Lane 1: PC-3 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
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Western Blot
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate
All lanes: Anxa1 antibody at 2.9µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
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Immunoprecipitating Anxa1 in NIH/3T3 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA001836YA01MO in NIH/3T3 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA001836YA01MO (8µg) + NIH/3T3 whole cell lysate (500µg)
Lane 3: NIH/3T3 whole cell lysate (20µg)
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Наименование: Anxa1 Antibody.