| Объем | 50 мкл |
| Синонимы | Aldo-keto reductase family 1 member C3 (EC 1.-.-.-) (17-beta-hydroxysteroid dehydrogenase type 5) (17-beta-HSD 5) (3-alpha-HSD type II, brain) (3-alpha-hydroxysteroid dehydrogenase type 2) (3-alpha-HSD type 2) (EC 1.1.1.357) (Chlordecone reductase homolog HAKRb) (Dihydrodiol dehydrogenase 3) (DD-3) (DD3) (Dihydrodiol dehydrogenase type I) (HA1753) (Indanol dehydrogenase) (EC 1.1.1.112) (Prostaglandin F synthase) (PGFS) (EC 1.1.1.188) (Testosterone 17-beta-dehydrogenase 5) (EC 1.1.1.239) (EC 1.1.1.64) (Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase) (EC 1.3.1.20), AKR1C3, DDH1 HSD17B5 KIAA0119 PGFS |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P42330 |
| Иммуноген | Recombinant Human Aldo-keto reductase family 1 member C3 protein (1-323AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Neuroscience, Cancer, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: AKR1C3 antibody at 8µg/ml
Lane 1: K562 whole cell lysate
Lane 2: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 37, 24 kDa
Observed band size: 37 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA001544ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human gallbladder tissue using CSB-PA001544ESR1HU at dilution of 1:100
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Immunoprecipitating AKR1C3 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA001544ESR1HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA001544ESR1HU (8µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (10µg)
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Наименование: AKR1C3 Antibody.